Genetic Characterization of Atypical Mansonella (Mansonella) ozzardi Microfilariae in Human Blood Samples from Northeastern Peru.

Marcos LA, Arrospide N, Recuenco S, Cabezas C, Weil GJ, Fischer PU.

Source

Infectious Diseases Division, Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri; Centro Nacional de Salud Pública, Instituto Nacional de Salud del Ministerio de Salud de Perú, Peru; Rabies Program, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia.

Abstract

DNA sequence comparisons are useful for characterizing proposed new parasite species or strains. Microfilariae with an atypical arrangement of nuclei behind the cephalic space have been recently described in human blood samples from the Amazon region of Peru. Three blood specimens containing atypical microfilariae were genetically characterized using three DNA markers (5S ribosomal DNA, 12S ribosomal DNA, and cytochrome oxidase I). All atypical microfilariae were clustered into the Mansonella group and indistinguishable from M. ozzardi based on these DNA markers.