Hepatitis E Virus and Porcine-derived Heparin

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To the Editor: Cases of sporadic, locally acquired hepatitis E have been increasingly identified in industrialized countries over the last few years (1). In this setting, hepatitis E is thought to be a zoonotic infection, with pigs as the primary host. Consumption of uncooked or lightly cooked pork meat products is thought to be a key route of infection, but other routes of transmission have been documented (2). For example, there have been several iatrogenic cases after transfusion of hepatitis E virus (HEV)–contaminated blood products (3) and transplantation of an HEV-infected donor liver (4). However, in most cases the source and route of infection are uncertain.
In May 2011, a 42-year-old woman sought care at the Royal Cornwall Hospital in Truro, United Kingdom, for a 1-week history of malaise, diarrhea, nausea, and vomiting. Physical examination results were normal. Her liver function test results, however, indicated hepatitis: alanine aminotransferase 2,785 IU/L (reference range 10–36 IU/L), alkaline phosphatase 319 IU/L (reference range 30–130 IU/L), and bilirubin 30 μmol/L (reference range <21 12="" 3="" 8="" a="" after="" an="" and="" as="" autoimmune="" b="" blood="" by="" c="" causes="" clinical="" epstein-barr="" excluded="" for="" function="" genotype="" hepatitis="" hepatocellular="" her="" hev="" identified="" igg="" igm="" immunocompetent="" in="" including="" jaundice="" liver="" made="" mol="" most="" normal="" of="" other="" p="" patient="" pcr="" persons="" positive="" recovery="" results="" returned="" reverse="" sequencing.="" serologic="" test="" testing.="" tests="" the="" to="" transcription="" uneventful="" viral="" virus="" viruses="" was="" weeks.="" weeks="" were="" with="">The source and route of infection in this case was uncertain. A detailed in-person assessment of potential risk factors was undertaken with the patient. She had not traveled outside the United Kingdom in the previous 3 months. She rarely ate pork products (well cooked bacon only); ate no shellfish; and had no workplace, domestic, or recreational exposure to pigs or their effluent. However, 4 weeks before symptom onset, the patient had acute appendicitis for which she underwent an uneventful laparoscopic appendectomy and was hospitalized for 2 days. During hospitalization she received no blood products, but, as prophylaxis for thromboembolic disease, she received 2 doses (5,000 IU each) of low–molecular weight heparin (Fragmin [dalteparin sodium]; Pfizer, Sandwich, UK) by subcutaneous injection. All heparins used in Europe and North America are isolated from porcine intestinal mucosa (5). The exact purification methods used by heparin manufacturers are deemed commercially sensitive and not in the public domain, so it is impossible to evaluate whether the isolation process would be sufficient to remove or inactivate any contaminating HEV. The virus is known to be acid and alkaline stable; heat sensitivity varies, depending on strain and heating conditions, although heating at 60°C for 1 hour is generally sufficient to achieve 96% inactivation (6). To our knowledge, no investigation has determined whether clinical-grade heparin could contain viral contaminants. Thus, we hypothesized that the heparin the patient received might have been the source of her HEV infection.
To examine this possibility, we screened multiple batches of hospital pharmacy–grade heparin for the presence of HEV, including batches of dalteparin sodium that were in use at the hospital when the patient received treatment for appendicitis. Before testing, the samples were ultracentrifuged to concentrate any contaminating virus and enable the removal of excipients, which could inhibit the assay. We tested samples by quantitative reverse transcription PCR (7) in parallel with positive World Health Organization HEV RNA standard spiked controls, which showed the limit of detection (LOD) to be 500 IU/mL, regardless of the heparin’s excipient or concentration. This LOD is within the range used by collaborating laboratories in the establishment of the World Health Organization HEV RNA standard (http://whqlibdoc.who.int/hq/2011/WHO_BS_2011.2175_eng.pdf

). In addition, we tested the heparin samples for porcine circovirus 2 (PCV2), an identified adventitious agent of several rotavirus vaccines (8) and porcine parvovirus (PPV) (9), a known contaminate of porcine clotting factor hyate:C (10). Although samples were tested in parallel with PCV2- and PPV- positive spiked controls, we were unable to calculate the LOD for these assays because international standards are not available for these viruses.
All samples tested negative for HEV, PCV2, and PPV (Table), which would indicate the patient’s source of HEV infection is unlikely to have been the heparin. However, we cannot rule out low-level viral contamination below the sensitivity of the assay. We also cannot exclude that the negative test results were related to the Poisson effect. Given that all samples analyzed were negative for all 3 viruses tested, it seems likely that the heparin manufacturing process is sufficient to remove viral contaminants. However, this may not necessarily be the case for other porcine-derived products, such as porcine insulin, factor VIII C, pancreatin, and poractant alfa. Further investigation is warranted to exclude these products as potential sources of HEV infection.

C. Crossan, L. Scobie, J. Godwin, J.G. Hunter, T. Hawkes, and H.R. Dalton

Author affiliations: Glasgow Caledonian University, Glasgow, Scotland, UK (C. Crossan, L. Scobie, J. Godwin); Royal Cornwall Hospital, Truro, UK (J.G. Hunter, T. Hawkes, H.R. Dalton); University of Exeter Medical School, Truro (H.R. Dalton)

Acknowledgment

We thank the Chief Scientist Office of Scotland who funded this work under project ETM/32.

References

Kamar N, Bendall R, Abravanel F, Xia N, Ijaz S, Izopet J, Hepatitis E. Lancet. 2012;379:2477–88. DOI PubMed
Scobie L, Dalton HR. Hepatitis E: source and route of infection, clinical manifestations and new developments. J Viral Hepat. 2013;20:1–11 . DOI PubMed
Colson P, Coze C, Gallian P, Henry M, De Micco P, Tamalet C. Transfusion-associated hepatitis E, France. Emerg Infect Dis. 2007;13:648–9. DOI PubMed
Schlosser B, Stein A, Neuhaus R, Paul S, Ramez B, Kruger DH, Liver transplant from a donor with occult HEV infection induced chronic hepatitis and cirrhosis in the recipient. J Hepatol. 2012;56:500–2. DOI PubMed
Alban S. The ‘precautionary principle’ as a guide for future drug development. Eur J Clin Invest. 2005;35(Suppl 1):33–44. DOI PubMed
Emerson SU, Arankalle VA, Purcell RH. Thermal stability of hepatitis E virus. J Infect Dis. 2005;192:930–3 . DOI PubMed
Jothikumar N, Cromeans TL, Robertson BH, Meng XJ, Hill VR. A broadly reactive one-step real-time RT-PCR assay for the rapid and sensitive detection of hepatitis E virus. J Virol Methods. 2006;131:65–71. DOI PubMed
Gilliland SM, Forrest L, Carre H, Jenkins A, Berry N, Martin J, Investigation of porcine circovirus in human vaccines. Biologicals. 2012;40:270–7 . DOI PubMed
Lau SKP, Woo PCY, Tse H, Fu CTY. Au Wk, Chen XC, et al. Identification of novel porcine and bovine parvoviruses closely related to human parvovirus 4. J Gen Virol. 2008;89:1840–8.
Soucie JM, Erdman DD, Evatt BL, Anderson LJ, Torok TJ, El-Jamil M, Investigation or porcine parvovirus among persons with hemophilia receiving Hyate:C porcine factor VIII concentrate. Transfusion. 2000;40:708–11. DOI PubMed

Table

Table. Heparin samples tested for hepatitis E virus, porcine circovirus 2, and porcine parvovirus

Suggested citation for this article: Crossan C, Scobie L, Godwin J, Hunter JG, Hawkes T, Dalton HR. Hepatitis E virus and porcine-derived heparin [letter]. Emerg Infect Dis [Internet]. 2013 April [date cited]. http://dx.doi.org/10.3201/eid1904.121792

DOI: 10.3201/eid1904.121792