lunes, 1 de septiembre de 2014

IL-32 is a molecular marker of a host defense network in human tuberculosis

IL-32 is a molecular marker of a host defense network in human tuberculosis



Sci Transl Med
Vol. 6, Issue 250, p. 250ra114 
Sci. Transl. Med. DOI: 10.1126/scitranslmed.3009546
  • RESEARCH ARTICLE
TUBERCULOSIS

IL-32 is a molecular marker of a host defense network in human tuberculosis

  1. Robert L. Modlin1,4,*
+Author Affiliations
  1. 1Division of Dermatology, David Geffen School of Medicine at University of California Los Angeles (UCLA), Los Angeles, CA 90095, USA.
  2. 2Department of Molecular, Cell, and Developmental Biology, UCLA, Los Angeles, CA 90095, USA.
  3. 3UCLA/Orthopaedic Hospital, Department of Orthopaedic Surgery, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.
  4. 4Department of Microbiology, Immunology and Molecular Genetics, UCLA, Los Angeles, CA 90095 USA.
  5. 5Department of Dermatology, University of Michigan School of Medicine, Ann Arbor, MI 48109, USA.
  6. 6Human Genetics, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.
  7. 7Biostatistics, School of Public Health, UCLA, Los Angeles, CA 90095, USA.
  8. 8Harvard School of Public Health, Boston, MA 02115, USA.
  1. *Corresponding author. E-mail: rmodlin@mednet.ucla.edu

Abstract

Tuberculosis is a leading cause of infectious disease–related death worldwide; however, only 10% of people infected with Mycobacterium tuberculosis develop disease. Factors that contribute to protection could prove to be promising targets for M. tuberculosis therapies. Analysis of peripheral blood gene expression profiles of active tuberculosis patients has identified correlates of risk for disease or pathogenesis. We sought to identify potential human candidate markers of host defense by studying gene expression profiles of macrophages, cells that, upon infection by M. tuberculosis, can mount an antimicrobial response. Weighted gene coexpression network analysis revealed an association between the cytokine interleukin-32 (IL-32) and the vitamin D antimicrobial pathway in a network of interferon-γ– and IL-15–induced “defense response” genes. IL-32 induced the vitamin D–dependent antimicrobial peptides cathelicidin and DEFB4 and to generate antimicrobial activity in vitro, dependent on the presence of adequate 25-hydroxyvitamin D. In addition, the IL-15–induced defense response macrophage gene network was integrated with ranked pairwise comparisons of gene expression from five different clinical data sets of latent compared with active tuberculosis or healthy controls and a coexpression network derived from gene expression in patients with tuberculosis undergoing chemotherapy. Together, these analyses identified eight common genes, including IL-32, as molecular markers of latent tuberculosis and the IL-15–induced gene network. As maintaining M. tuberculosis in a latent state and preventing transition to active disease may represent a form of host resistance, these results identify IL-32 as one functional marker and potential correlate of protection against active tuberculosis.
Citation: D. Montoya, M. S. Inkeles, P. T. Liu, S. Realegeno, R. M. B. Teles, P. Vaidya, M. A. Munoz, M. Schenk, W. R. Swindell, R. Chun, K. Zavala, M. Hewison, J. S. Adams, S. Horvath, M. Pellegrini, B. R. Bloom, R. L. Modlin, IL-32 is a molecular marker of a host defense network in human tuberculosis. Sci. Transl. Med. 6250ra114 (2014).


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