Ahead of Print -Distinct Lineages of Bufavirus in Wild Shrews and Nonhuman Primates - Volume 21, Number 7—July 2015 - Emerging Infectious Disease journal - CDC

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Ahead of Print -Distinct Lineages of Bufavirus in Wild Shrews and Nonhuman Primates - Volume 21, Number 7—July 2015 - Emerging Infectious Disease journal - CDC

Volume 21, Number 7—July 2015

Dispatch

Distinct Lineages of Bufavirus in Wild Shrews and Nonhuman Primates

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• The Study
• Conclusions
• Suggested Citation

Figures

• Figure

Tables

• Table

Technical Appendicies

• Technical Appendix

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Michihito Sasaki, Yasuko Orba, Paulina D. Anindita, Akihiro Ishii, Keisuke Ueno1, Bernard M. Hang’ombe, Aaron S. Mweene, Kimihito Ito, and Hirofumi Sawa 

Author affiliations: Hokkaido University, Sapporo, Japan (M. Sasaki, Y. Orba, P.D. Anindita, A. Ishii, K. Ueno, K. Ito, H. Sawa); University of Zambia, Lusaka, Zambia (B.M. Hang’ombe, A.S. Mweene)

Suggested citation for this article

Abstract

Viral metagenomic analysis identified a new parvovirus genome in the intestinal contents of wild shrews in Zambia. Related viruses were detected in spleen tissues from wild shrews and nonhuman primates. Phylogenetic analyses showed that these viruses are related to human bufaviruses, highlighting the presence and genetic diversity of bufaviruses in wildlife.

Bufavirus (BuV), a recently described parvovirus, was initially discovered in the feces of a child with diarrhea in Burkina Faso in 2012 (1). Thereafter, BuV was identified in fecal samples from children and adults with gastroenteritis in Bhutan, Finland, and the Netherlands in 2014 (2–4), respectively. Genome sequences and phylogenetic analyses revealed that BuV comprised at least 3 genotypes and was distinct from all other known members of the Parvoviridae family (1,2). The International Committee on Taxonomy of Viruses assigned BuV as a new species of the genus Protoparvovirus in the subfamily Parvovirinae (5). Whether BuV is an etiologic agent of human gastroenteritis remains unclear, but knowledge about its distribution and genetic divergence in humans is accumulating. However, whether BuV infection exists in wildlife remains unanswered. Through use of metagenomics, we previously described the enteric virome of wild shrews of the Crocidura genus sampled at Mpulungu, Zambia, in 2012 (6). From this sequence dataset (GenBank/EMBL/DDBJ accession no. DRA002561), we identified sequence reads related to BuV. Here, we describe the genome of this new parvovirus.

Dr. Sasaki is a veterinary researcher at the Research Center for Zoonosis Control, Hokkaido University, and is certified as a zoonosis control expert by Hokkaido University. His research interests include the molecular basis of viral pathogenesis.

Acknowledgments

We thank the Zambia Wildlife Authority for its support with this research.

This work was supported by the Global Institution for Collaborative Research and Education (GI-CoRE) and the Japan Initiative for Global Research Network of Infectious Diseases (J-GRID) from the Japan Ministry of Education, Culture, Sports, Science, and Technology and Grants-in-Aid for Scientific Research (KAKENHI, grant no. 24405043) from the Japan Society for the Promotion of Science.

References

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Figure

• Figure. Partial nonstructural protein (NS) 1 gene phylogeny of newly identified bufaviruses, Zambia. The Bayesian phylogenetic tree was generated by using the partial NS1 gene fragments (434–440 bp) of bufaviruses and...

Table

• Table. Sample information and nested PCR screening results for bufavirus, Zambia

Technical Appendix

• Technical Appendix. Genome organization and phylogeny of Mpulungu bufavirus.  164 KB

Suggested citation for this article: Sasaki M, Orba Y, Anindita PD, Ishii A, Ueno K, Hang’ombe BM, et al. Distinct lineages of bufavirus in wild shrews and nonhuman primates. Emerg Infect Dis. 2015 Jul [date cited]. http://dx.doi.org/10.3201/eid2107.141969

DOI: 10.3201/eid2107.141969

1Current affiliation: King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.