domingo, 4 de diciembre de 2016

Metagenomics of two severe foodborne outbreaks provides diagnostic signatures and signs of co-infection not attainable by traditional methods. - PubMed - NCBI

Metagenomics of two severe foodborne outbreaks provides diagnostic signatures and signs of co-infection not attainable by traditional methods. - PubMed - NCBI

 2016 Nov 23. pii: AEM.02577-16. [Epub ahead of print]

Metagenomics of two severe foodborne outbreaks provides diagnostic signatures and signs of co-infection not attainable by traditional methods.

Abstract

Diagnostic testing for foodborne pathogens relies on culture-based techniques that are not rapid enough for real-time disease surveillance and do not give a quantitative picture of pathogen abundance or the response of the natural microbiome. Powerful sequence-based, culture-independent approaches such as shotgun metagenomics could sidestep these limitations, and potentially reveal a pathogen-specific signature on the microbiome that would have implications not only for diagnostics but also for better understanding disease progression and pathogen ecology. However, metagenomics have not yet been validated for foodborne pathogen detection. Toward closing these gaps, we applied shotgun metagenomics to stool samples collected from two geographically isolated (Alabama and Colorado) foodborne outbreaks, where the etiologic agents were identified as distinct strains of Salmonella enterica serovar Heidelberg by culture-dependent methods. Metagenomic investigations were consistent with the culture-based findings and revealed, in addition, the in-situ abundance and level of intra-population diversity of the pathogen, the possibility for co-infections with Staphylococcus aureus, and significant shifts in the gut microbiome during infection relative to reference healthy samples. Additionally, we designed our bioinformatics pipeline to deal with several challenges associated with analysis of clinical samples such as the high frequency of co-eluting human DNA sequences and assessment of the virulence potential of pathogens. Comparisons of these results to those of other studies revealed that in several cases of diarrheal outbreaks -but not all- the disease and healthy states of the gut microbial community might be distinguishable, opening new possibilities for diagnostics.

IMPORTANCE STATEMENT:

Diagnostic testing for enteric pathogens has relied for decades on culture-based techniques but a total of 38.4 million cases of foodborne illness per year cannot be attributed to specific causes. This study describes new culture-independent metagenomic approaches and the associated bioinformatics approaches to detect and type the causative agents of microbial disease with unprecedented accuracy, opening new possibilities for future development of health technologies and diagnostics. Our tools and approaches should be applicable to other microbial diseases in addition to foodborne diarrhea.
PMID:
 
27881416
 
DOI:
 
10.1128/AEM.02577-16
[PubMed - as supplied by publisher]

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