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1-methylnicotinamide and its structural analog 1,4-dimethylpyridine for the prevention of cancer metastasis | Journal of Experimental & Clinical Cancer Research | Full Text

1-methylnicotinamide and its structural analog 1,4-dimethylpyridine for the prevention of cancer metastasis | Journal of Experimental & Clinical Cancer Research | Full Text

Biomed Central



Journal of Experimental & Clinical Cancer Research

1-methylnicotinamide and its structural analog 1,4-dimethylpyridine for the prevention of cancer metastasis

  • Agnieszka Blazejczyk,
  • Marta Switalska,
  • Stefan Chlopicki,
  • Andrzej Marcinek,
  • Jerzy Gebicki,
  • Marcin Nowak,
  • Anna Nasulewicz-Goldeman and
  • Joanna WietrzykEmail author
Journal of Experimental & Clinical Cancer Research201635:110
DOI: 10.1186/s13046-016-0389-9
Received: 26 April 2016
Accepted: 28 June 2016
Published: 13 July 2016



Abstract

Background

1-methylnicotinamide (1-MNA), an endogenous metabolite of nicotinamide, has recently gained interest due to its anti-inflammatory and anti-thrombotic activities linked to the COX-2/PGI2 pathway. Given the previously reported anti-metastatic activity of prostacyclin (PGI2), we aimed to assess the effects of 1-MNA and its structurally related analog, 1,4-dimethylpyridine (1,4-DMP), in the prevention of cancer metastasis.

Methods

All the studies on the anti-tumor and anti-metastatic activity of 1-MNA and 1,4-DMP were conducted using the model of murine mammary gland cancer (4T1) transplanted either orthotopically or intravenously into female BALB/c mouse. Additionally, the effect of the investigated molecules on cancer cell-induced angiogenesis was estimated using the matrigel plug assay utilizing 4T1 cells as a source of pro-angiogenic factors.

Results

Neither 1-MNA nor 1,4-DMP, when given in a monotherapy of metastatic cancer, influenced the growth of 4T1 primary tumors transplanted orthotopically; however, both compounds tended to inhibit 4T1 metastases formation in lungs of mice that were orthotopically or intravenously inoculated with 4T1 or 4T1-luc2-tdTomato cells, respectively. Additionally, while 1-MNA enhanced tumor vasculature formation and markedly increased PGI2 generation, 1,4-DMP did not have such an effect. The anti-metastatic activity of 1-MNA and 1,4-DMP was further confirmed when both agents were applied with a cytostatic drug in a combined treatment of 4T1 murine mammary gland cancer what resulted in up to 80 % diminution of lung metastases formation.

Conclusions

The results of the studies presented below indicate that 1-MNA and its structural analog 1,4-DMP prevent metastasis and might be beneficially implemented into the treatment of metastatic breast cancer to ensure a comprehensive strategy of metastasis control.

Keywords

1-methylnicotinaimide chloride 1,4-dimethylpyridinium chloride Metastasis Angiogenesis Combined therapy Breast cancer

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