domingo, 12 de marzo de 2017

Pin1 and secondary hyperparathyroidism of chronic kidney disease: gene polymorphisms and protein levels. - PubMed - NCBI

Pin1 and secondary hyperparathyroidism of chronic kidney disease: gene polymorphisms and protein levels. - PubMed - NCBI



 2017 Nov;39(1):159-165. doi: 10.1080/0886022X.2016.1256310. Epub 2016 Nov 23.

Pin1 and secondary hyperparathyroidism of chronic kidney disease: gene polymorphisms and protein levels.

Abstract

BACKGROUND:

Peptidyl-prolyl cis/trans isomerase NIMA-interacting 1 (Pin1) is a key regulator of PTH mRNA stability. Secondary hyperparathyroidism (SHPT), which is characterized by elevated serum PTH levels, is a common complication of CKD. We investigated the possible associations between CKD with SHPT (CKD SHPT) and single-nucleotide polymorphisms of the Pin1 gene and compared the levels of the Pin1 protein in the CKD SHPT patients with those of the controls.

METHODS:

The study group included 251 CKD SHPT patients and 61 controls. One putative functional SNP (single nucleotide polymorphism) in the Pin1 promoter (rs2233679C > T: c.-667C > T) is the main object. Genotyping was performed on purified DNA using polymerase chain reaction-restriction (PCR) and restriction fragment length polymorphisms (RFLP). The levels of Pin1 were measured in serum using an enzyme-linked immunosorbent assay.

RESULTS:

Genotyping showed that CT + TT in the Pin1 promoter was significantly more common in the CKD SHPT group than in the control group (p<.05). The correlation analysis demonstrated that a significant difference in the C to T transition in the Pin1 promoter contributed to CKD SHPT (χ2=12.47, p<.05; Odds ratios (OR) = 1.26, 95% confidence (CI) intervals =1.06-1.49). The multivariate logistic regression analysis reported that the OR and 95%CI were 12.693 and 2.029-75.819 (p<.05), respectively, in the Pin1 gene promoter -667T variant genotypes (CT + TT) after adjusting for other factors, and those values in Pin1 were 0.310 and 0.122-0.792 (p<.05).

CONCLUSION:

The -667T genetic variants in the Pin1 promoter contribute to an increased risk of CKD SHPT and may be biomarkers of susceptibility to CKD SHPT.

KEYWORDS:

Chronic kidney disease; peptidyl-prolyl cis/trans isomerase NIMA-interacting 1; secondary hyperparathyroidism; single nucleotide polymorphism

PMID:
 
27876426
 
DOI:
 
10.1080/0886022X.2016.1256310


https://phgkb.cdc.gov/GAPPKB/phgHome.do



Last Posted: Mar-10-2017 9AM
a body with  kidneys



From HuGE Literature Finder Database
This database contains published literature on genetic associations and other human genome epidemiology



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